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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using BLZF1 Polyclonal antibody (16354-1-AP)

    WB analysis of K-562 using 16354-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BLZF1 Polyclonal antibody (16354-1-AP)

    WB analysis of Jurkat using 16354-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of human liver cancer using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of human liver cancer using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of mouse liver using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BLZF1 Polyclonal antibody (16354-1-AP)

    IHC staining of mouse liver using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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BLZF1 Polyclonal antibody

BLZF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16354-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BLZF1, GOLGIN 45, JEM 1, JEM 1s, JEM1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16354-1-AP targets BLZF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLZF1 fusion protein Ag9564 相同性解析による交差性が予測される生物種
Full Name basic leucine zipper nuclear factor 1
Calculated molecular weight 400 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC020716
Gene Symbol BLZF1
Gene ID (NCBI) 8548
RRIDAB_2918045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.

Protocols

Product Specific Protocols
WB protocol for BLZF1 antibody 16354-1-APDownload protocol
IHC protocol for BLZF1 antibody 16354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Biol Cell

Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.

Authors - Michael E Bekier
View Article
humanWB

Methods Mol Biol

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus

Authors - Maryam Arab
View Article
WB

Methods Mol Biol

Common Markers and Small Molecule Inhibitors in Golgi Studies

Authors - Sarah Bui
View Article