Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
18982-1-AP targets BMP15 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | bone morphogenetic protein 15 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | NM_005448 |
| Gene Symbol | BMP15 |
| Gene ID (NCBI) | 9210 |
| RRID | AB_2878570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BMP15, also named as GDF9B, is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. BMP15 may be involved follicular development and oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Mutation of BMP15 will cause of ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BMP15 antibody 18982-1-AP | Download protocol |
| IHC protocol for BMP15 antibody 18982-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Commun Signal GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement | ||
Mol Med Rep Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome. | ||
Proc Natl Acad Sci U S A A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human | ||
Adv Sci (Weinh) AMPK Suppression Due to Obesity Drives Oocyte mtDNA Heteroplasmy via ATF5-POLG Axis | ||
Environ Toxicol Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse | ||
Reprod Sci Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian Damage Caused by Chronic Unpredictable Mild Stress Through the eIF2α-AFT4 Signaling Pathway in Mice |