BMP2 Monoclonal antibody
BMP2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig and More (2)
Applications
WB, IHC, IF, ELISA, Cell treatment
Conjugate
Unconjugated
CloneNo.
1C5C2
Cat no : 66383-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig bone marrow tissue, Caco-2 cells, HeLa cells, pig aorta tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 39 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
Product Information
66383-1-Ig targets BMP2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BMP2 fusion protein Ag13501 相同性解析による交差性が予測される生物種 |
| Full Name | bone morphogenetic protein 2 |
| Calculated molecular weight | 396 aa, 44 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC069214 |
| Gene symbol | BMP2 |
| Gene ID (NCBI) | 650 |
| RRID | AB_2881759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| IHC protocol for BMP2 antibody 66383-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions. | ||
Angiogenesis Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8. | ||
Br J Pharmacol Andrographolide regulates H3 histone lactylation by interfering with p300 to alleviate aortic valve calcification | ||
Phytother Res Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes | ||
Oncogenesis Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway. |
