BMP3 Polyclonal antibody
BMP3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 22188-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Positive IHC detected in | human liver cancer tissue, human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 22188-1-AP is BMP3 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BMP3 fusion protein Ag16750 相同性解析による交差性が予測される生物種 |
Full Name | bone morphogenetic protein 3 |
Calculated molecular weight | 472 aa, 53 kDa |
GenBank accession number | BC096269 |
Gene symbol | BMP3 |
Gene ID (NCBI) | 651 |
RRID | AB_2879019 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.
Protocols
Product Specific Protocols | |
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WB protocol for BMP3 antibody 22188-1-AP | Download protocol |
IHC protocol for BMP3 antibody 22188-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biol Trace Elem Res Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells. |