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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BMP5 Polyclonal antibody

BMP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13253-1-AP

Synonyms

BMP 5, BMP5, BMP-5, bone morphogenetic protein 5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of mouse lung using 13253-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of mouse lung using 13253-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using BMP5 Polyclonal antibody (13253-1-AP)

    WB analysis of HUVEC using 13253-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 13253-1-AP (BMP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using BMP5 Polyclonal antibody (13253-1-AP)

    IHC staining of human colon using 13253-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13253-1-AP (BMP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, HUVEC cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13253-1-AP targets BMP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMP5 fusion protein Ag3952 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein 5
Calculated molecular weight 454 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC027958
Gene symbol BMP5
Gene ID (NCBI) 653
RRIDAB_2877929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BMP5 antibody 13253-1-APDownload protocol
IHC protocol for BMP5 antibody 13253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

G9a Knockdown Suppresses Cancer Aggressiveness by Facilitating Smad Protein Phosphorylation through Increasing BMP5 Expression in Luminal A Type Breast Cancer.

Authors - Yunho Jin
View Article
humanWB

FASEB J

Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis

Authors - Xiang Gao
View Article
humanIHC

Mol Cancer

Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.

Authors - Erfei Chen
View Article