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Validation Data Gallery

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  • Western Blot (WB) analysis of pig lung tissue using BMP5 Monoclonal antibody (66370-1-Ig)

    WB analysis of pig lung using 66370-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66370-1-Ig (BMP5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using BMP5 Monoclonal antibody (66370-1-Ig)

    WB analysis of pig liver using 66370-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66370-1-Ig (BMP5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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BMP5 Monoclonal antibody

BMP5 Monoclonal Antibody for WB, ELISA
Cat No. 66370-1-Ig
Clone No.1G6C10

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

BMP 5, BMP5, bone morphogenetic protein 5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig lung tissue, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66370-1-Ig targets BMP5 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BMP5 fusion protein Ag3952 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein 5
Calculated molecular weight 454 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC027958
Gene Symbol BMP5
Gene ID (NCBI) 653
RRIDAB_2881749
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22003
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bone morphogenetic protein-5 (BMP5), is a member of the TGF-beta superfamily. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. BMP5 may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. BMP5 is differentially regulated in multiple human cancers.

Protocols

Product Specific Protocols
WB protocol for BMP5 antibody 66370-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols