Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 12702-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12702-1-AP
WB result of BMPR1A antibody (12702-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMPR1A transfected MCF-7 cells.
WB analysis of human skeletal muscle using 12702-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12702-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12702-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12702-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12702-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12702-1-AP (BMPR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12702-1-AP
IP result of anti-BMPR1A (IP:12702-1-AP, 4ug; Detection:12702-1-AP 1:500) with HEK-293 cells lysate 2000ug.
IF Staining of U2OS using 12702-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using BMPR1A antibody (12702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12702-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ALK-3, ALK3, ALK 3, ACVRLK3, Activin receptor-like kinase 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, human heart tissue, human skeletal muscle tissue, K-562 cells, MCF-7 cells, mouse liver tissue, Jurkat cells, U2OS cells
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

12702-1-AP targets BMPR1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BMPR1A fusion protein Ag3410 相同性解析による交差性が予測される生物種
Full Name	bone morphogenetic protein receptor, type IA
Calculated molecular weight	532 aa, 60 kDa
Observed molecular weight	60-68 kDa
GenBank accession number	BC028383
Gene Symbol	BMPR1A
Gene ID (NCBI)	657
RRID	AB_2064273
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36894
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BMPR1A antibody 12702-1-AP	Download protocol
IF protocol for BMPR1A antibody 12702-1-AP	Download protocol
IP protocol for BMPR1A antibody 12702-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Biol A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation. Authors - Ling Guo View Article
human	IP	Sci Signal Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells. Authors - Sreeharsha Gurrapu View Article
mouse	WB,IF	Front Cell Dev Biol Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland. Authors - Chunlei Shao View Article
mouse	WB	PLoS One Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro. Authors - Shasha Zhu View Article
human	WB	Biochim Biophys Acta Gen Subj Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels Authors - Tianmiao Huang View Article
rat	WB	Sci Rep Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells. Authors - Yan Zeng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12702-1-AP

WB analysis of MCF-7 using 12702-1-AP

WB analysis of human skeletal muscle using 12702-1-AP

WB analysis of human heart using 12702-1-AP

WB analysis of HEK-293 using 12702-1-AP

WB analysis of K-562 using 12702-1-AP

WB analysis of mouse liver using 12702-1-AP

IP experiment of HEK-293 using 12702-1-AP

IF Staining of U2OS using 12702-1-AP

BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.

Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells.

Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.

Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro.

Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels

Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.