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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BMPR1B Polyclonal antibody (26696-1-AP)

    WB analysis using 26696-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26696-1-AP (BMPR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BMPR1B Polyclonal antibody (26696-1-AP)

    IF Staining of MCF-7 using 26696-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BMPR1B antibody (26696-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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BMPR1B Polyclonal antibody

BMPR1B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26696-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ALK 6, ALK6, BMP type 1B receptor, BMPR 1B, BMPR1B, CDw293

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26696-1-AP targets BMPR1B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMPR1B fusion protein Ag24902 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type IB
Calculated molecular weight 57 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC047773
Gene Symbol BMPR1B
Gene ID (NCBI) 658
RRIDAB_3085892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMPR1B (Bone morphogenetic protein receptor type-1B) is also named as CDw293. BMPR1B belongs to the protein kinase superfamily. BMPR1B is on ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. BMPR1B is receptor for BMP7/OP-1 and GDF5. BMPR1B Positively regulates chondrocyte differentiation through GDF5 interaction.

Protocols

Product Specific Protocols
WB protocol for BMPR1B antibody 26696-1-APDownload protocol
IF protocol for BMPR1B antibody 26696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurooncol

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells

Authors - Hui Xu
View Article