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  • Featured Product
  • KD/KO Validated

BMPR2 Polyclonal antibody, PBS Only

BMPR2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19087-1-PBS

Synonyms

BMPR-2, BMPR II, BMPR 2, BMP type-2 receptor, BMP type II receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    WB analysis of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    WB analysis of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IP experiment of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    IP result of anti-BMPR2 (IP:19087-1-AP, 4ug; Detection:19087-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BMPR2 Polyclonal antibody (19087-1-AP)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using BMPR2 Polyclonal antibody (19087-1-AP)

    FC experiment of PC-3 using 19087-1-AP (same clone as 19087-1-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug BMPR2 Polyclonal antibody (19087-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19087-1-PBS targets BMPR2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMPR2 fusion protein Ag5868 相同性解析による交差性が予測される生物種
Full Name bone morphogenetic protein receptor, type II (serine/threonine kinase)
Calculated molecular weight 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC052985
Gene symbol BMPR2
Gene ID (NCBI) 659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. The dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form, and 150 kD dimer.