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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BNIP1 Polyclonal antibody, PBS Only

BNIP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15964-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BNIP1 Polyclonal antibody (15964-1-AP)

    WB analysis using 15964-1-AP (same clone as 15964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15964-1-AP (BNIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IP experiment of mouse brain using 15964-1-AP (same clone as 15964-1-PBS)

    IP result of anti-BNIP1 (IP:15964-1-AP, 3ug; Detection:15964-1-AP 1:500) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using BNIP1 Polyclonal antibody (15964-1-AP)

    IHC staining of human heart using 15964-1-AP (same clone as 15964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15964-1-AP (BNIP1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15964-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15964-1-PBS targets BNIP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNIP1 fusion protein Ag8737 相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 1
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC010959
Gene symbol BNIP1
Gene ID (NCBI) 662
RRIDAB_2066636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.