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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BNIP2 Polyclonal antibody (10361-1-AP)

    WB analysis using 10361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10361-1-AP (BNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BNIP2 Polyclonal antibody (10361-1-AP)

    IF Staining of HeLa using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using BNIP2 Polyclonal antibody (10361-1-AP)

    IF Staining of RAW 264.7 using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

BNIP2 Polyclonal antibody

BNIP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10361-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

NIP2, BNIP 2, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, MCF-7 cells, MDA-MB-231 cells, SW480 cells
Positive IF/ICC detected inHeLa cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10361-1-AP targets BNIP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BNIP2 fusion protein Ag0403 相同性解析による交差性が予測される生物種
Full Name BCL2/adenovirus E1B 19kDa interacting protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002461
Gene Symbol BNIP2
Gene ID (NCBI) 663
RRIDAB_3085366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12982
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) is also named as PRKACN2. BNIP2 is developmentally regulated may suggest a role of this gene in those brain areas where the differentiation is orchestrated by estradiol. The expression of BNIP2 was inhibited by estradiol. BNIP2 is implicated in the suppression of cell death and interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. BNIP2 is highly expressed in patients with favorable neuroblastoma (NB) (PMID: 25611382).

Protocols

Product Specific Protocols
WB protocol for BNIP2 antibody 10361-1-APDownload protocol
IF protocol for BNIP2 antibody 10361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols