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- KD/KO Validated
BNIP3L Polyclonal antibody
BNIP3L Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12986-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Raji cells, mouse kidney tissue, Jurkat cells, rat kidney tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 17 publications below |
| IHC | See 3 publications below |
Product Information
12986-1-AP targets BNIP3L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BNIP3L fusion protein Ag3624 相同性解析による交差性が予測される生物種 |
| Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3-like |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC001559 |
| Gene symbol | BNIP3L |
| Gene ID (NCBI) | 665 |
| RRID | AB_2877901 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BNIP3L, also named as BNIP3A, BNIP3H, NIX and NIP3L. Bnip3L is one of the BH3-containing pro-apoptotic proteins and that it targets the mitochondria when inducing apoptosis. It may function as a tumor suppressor. BNIP3L has Dimer form with MW ~70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BNIP3L antibody 12986-1-AP | Download protocol |
| IHC protocol for BNIP3L antibody 12986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. | ||
Autophagy Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.
| ||
Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells | ||
Neuromuscul Disord Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase autoantibodies: ultrastructural and immunohistochemical studies. | ||
Int J Mol Sci In Vivo Metabolic Responses to Different Formulations of Amino Acid Mixtures for the Treatment of Phenylketonuria (PKU). |
