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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BOP1 Recombinant antibody (82742-3-RR)

    WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BOP1 Recombinant antibody (82742-3-RR)

    WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using BOP1 Recombinant antibody (82742-3-RR)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using BOP1 Recombinant antibody (82742-3-RR)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BOP1 Recombinant antibody (82742-3-RR)

    IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BOP1 Recombinant antibody (82742-3-RR)

    IF Staining of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

BOP1 Recombinant antibody, PBS Only

BOP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82742-3-PBS
Clone No.3A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0124, block of proliferation 1, 3A4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82742-3-PBS targets BOP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BOP1 fusion protein Ag26669 相同性解析による交差性が予測される生物種
Full Name block of proliferation 1
Calculated molecular weight 84 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC013787
Gene Symbol BOP1
Gene ID (NCBI) 23246
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14137
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).