BPGM Polyclonal antibody

Name: BPGM Polyclonal antibody
Brand: Proteintech
SKU: 17173-1-AP

BPGM Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17173-1-AP

Synonyms

2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse liver using 17173-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 17173-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 17173-1-AP
human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 17173-1-AP
human blood were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17173-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17173-1-AP (BPGM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 17173-1-AP
IP result of anti-BPGM (IP:17173-1-AP, 4ug; Detection:17173-1-AP 1:700) with mouse liver tissue lysate 6000 ug.
IHC staining of human liver cancer using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 17173-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17173-1-AP (BPGM Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 17173-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

17173-1-AP targets BPGM in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BPGM fusion protein Ag10731 相同性解析による交差性が予測される生物種
Full Name	2,3-bisphosphoglycerate mutase
Calculated molecular weight	259 aa, 30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC017050
Gene symbol	BPGM
Gene ID (NCBI)	669
RRID	AB_2274991
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BPGM antibody 17173-1-AP	Download protocol
IHC protocol for BPGM antibody 17173-1-AP	Download protocol
IP protocol for BPGM antibody 17173-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IP	Cell Metab Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis. Authors - Ping Xu View Article
rat	WB,IF	Brain Res Bull Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Authors - Guoji E View Article
rat	WB	Front Physiol Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia Authors - Song Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BPGM Polyclonal antibody

BPGM Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse liver using 17173-1-AP

WB analysis of rat liver using 17173-1-AP

WB analysis of human plasma using 17173-1-AP

WB analysis of human plasma using 17173-1-AP

WB analysis of mouse liver using 17173-1-AP

IP experiment of mouse liver using 17173-1-AP

IHC staining of human liver cancer using 17173-1-AP

IHC staining of human liver cancer using 17173-1-AP

IHC staining of human liver using 17173-1-AP