BPIL1 Polyclonal antibody

Name: BPIL1 Polyclonal antibody
Brand: Proteintech
SKU: 13461-2-AP

BPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13461-2-AP

Synonyms

Long palate, lung and nasal epithelium carcinoma-associated protein 2, C20orf184, BPI-like 1, BPIFB2, BPI fold-containing family B member 2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13461-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 13461-2-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13461-2-AP (BPIL1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13461-2-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.
IHC staining of human tonsillitis using 13461-2-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13461-2-AP (BPIL1 Antibody) at dilution of 1:50.
IF Staining of A549 using 13461-2-AP
Immunofluorescent analysis of A549 cells using 13461-2-AP (BPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 13461-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 2 publications below

Product Information

13461-2-AP targets BPIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BPIL1 fusion protein Ag4051 相同性解析による交差性が予測される生物種
Full Name	bactericidal/permeability-increasing protein-like 1
Calculated molecular weight	458 aa, 49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC034415
Gene symbol	BPIL1
Gene ID (NCBI)	80341
RRID	AB_2067089
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein which may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).

Protocols

Product Specific Protocols
WB protocol for BPIL1 antibody 13461-2-AP	Download protocol
IHC protocol for BPIL1 antibody 13461-2-AP	Download protocol
IF protocol for BPIL1 antibody 13461-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Allergy Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis. Authors - Seshadri S S View Article
human	WB	J Proteome Res Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure. Authors - Foster Matthew W MW View Article
human	IHC	J Immunol Res Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps. Authors - Yanran Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BPIL1 Polyclonal antibody

BPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

WB analysis of A549 using 13461-2-AP

IHC staining of human tonsillitis using 13461-2-AP

IHC staining of human tonsillitis using 13461-2-AP

IHC staining of human tonsillitis using 13461-2-AP

IF Staining of A549 using 13461-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.

Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.