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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRAP Polyclonal antibody

BRAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26004-1-AP

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BRAP Polyclonal antibody (26004-1-AP)

    WB analysis of MCF-7 using 26004-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26004-1-AP (BRAP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRAP Polyclonal antibody (26004-1-AP)

    IHC staining of human kidney using 26004-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRAP Polyclonal antibody (26004-1-AP)

    IHC staining of human kidney using 26004-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26004-1-AP (BRAP Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26004-1-AP is BRAP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAP fusion protein Ag23279 相同性解析による交差性が予測される生物種
Full Name BRCA1 associated protein
Calculated molecular weight 592 aa, 67 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC136698
Gene symbol BRAP
Gene ID (NCBI) 8315
RRIDAB_2880330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BRAP antibody 26004-1-APDownload protocol
IHC protocol for BRAP antibody 26004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols