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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BRAP Polyclonal antibody (27431-1-AP)

    WB analysis of MCF-7 using 27431-1-AP (same clone as 27431-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using BRAP Polyclonal antibody (27431-1-AP)

    WB analysis of A431 using 27431-1-AP (same clone as 27431-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 27431-1-AP (BRAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using BRAP Polyclonal antibody (27431-1-AP)

    IP experiment of MCF-7 using 27431-1-AP (same clone as 27431-1-PBS)

    IP result of anti-BRAP (IP:27431-1-AP, 4ug; Detection:27431-1-AP 1:1000) with MCF-7 cells lysate 3200 ug. This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human lung cancer using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human lung cancer using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of mouse testis using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of mouse testis using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human breast cancer using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BRAP Polyclonal antibody (27431-1-AP)

    IHC staining of human breast cancer using 27431-1-AP (same clone as 27431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27431-1-AP (BRAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27431-1-PBS in a different storage buffer formulation.

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BRAP Polyclonal antibody, PBS Only

BRAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27431-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27431-1-PBS targets BRAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAP fusion protein Ag26728 相同性解析による交差性が予測される生物種
Full Name BRCA1 associated protein
Calculated molecular weight 592 aa, 67 kDa
Observed molecular weight 62-70 kDa, 48 kDa
GenBank accession numberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
RRIDAB_2880869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z569
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.