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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRAP Recombinant antibody

BRAP Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240099D8

Cat no : 83314-6-RR

Synonyms

BRAP, BRAP2, BRCA1 associated protein, IMP, RING finger protein 52, RNF52

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BRAP Recombinant antibody (83314-6-RR)

    IF Staining of MCF-7 using 83314-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity experiments of 83314-6-RR

    Affinity experiments of 83314-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83314-6-RR is BRAP Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BRAP fusion protein Ag26728 相同性解析による交差性が予測される生物種
Full Name BRCA1 associated protein
Calculated molecular weight 592 aa, 67 kDa
GenBank accession numberBC136698
Gene symbol BRAP
Gene ID (NCBI) 8315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for BRAP antibody 83314-6-RRDownload protocol
FC protocol for BRAP antibody 83314-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols