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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRAP Recombinant antibody

BRAP Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240099D8

Cat no : 83314-6-RR

Synonyms

Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BRAP Recombinant antibody (83314-6-RR)

    IF Staining of MCF-7 using 83314-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HeLa cells using BRAP Recombinant antibody (83314-6-RR)

    FC experiment of HeLa using 83314-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BRAP Recombinant antibody (83314-6-RR, Clone:240099D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83314-6-RR

    Affinity and Kinetic Characterization of 83314-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is 6.34 nM.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83314-6-RR is BRAP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BRAP fusion protein Ag26728 相同性解析による交差性が予測される生物種
Full Name BRCA1 associated protein
Calculated molecular weight 592 aa, 67 kDa
GenBank accession numberBC136698
Gene symbol BRAP
Gene ID (NCBI) 8315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for BRAP antibody 83314-6-RRDownload protocol
FC protocol for BRAP antibody 83314-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols