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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRCA1 Recombinant antibody, PBS Only (Capture/Detector)

BRCA1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240285H6

Cat no : 83390-2-PBS

Synonyms

PSCP, IRIS, EC:2.3.2.27, BROVCA1, BRCC1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00425-2

    Sandwich ELISA standard curve of MP00425-2

    Sandwich ELISA standard curve of MP00425-2, Human BRCA1 Recombinant Matched Antibody Pair - PBS only. 83390-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17946. 83390-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP00425-2

    Sample test of MP00425-2

    The mean BRCA1 concentration was determined to be 6.24 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 5.16 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 5.29 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP00425-2

    Cytometric bead array standard curve of MP00425-2

    Cytometric bead array standard curve of MP00425-2, BRCA1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83390-4-PBS. Detection antibody: 83390-2-PBS. Standard: Ag17946. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00425-3

    Cytometric bead array standard curve of MP00425-3

    Cytometric bead array standard curve of MP00425-3, BRCA1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83390-2-PBS. Detection antibody: 83390-1-PBS. Standard: Ag17946. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83390-2-PBS targets BRCA1 as part of a matched antibody pair:

MP00425-2: 83390-4-PBS capture and 83390-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00425-3: 83390-2-PBS capture and 83390-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BRCA1 fusion protein Ag17946 相同性解析による交差性が予測される生物種
Full Name breast cancer 1, early onset
Calculated molecular weight 1863 aa, 208 kDa
GenBank accession numberBC115037
Gene symbol BRCA1
Gene ID (NCBI) 672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.