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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRD3 Polyclonal antibody (11859-1-AP)

    WB analysis using 11859-1-AP (same clone as 11859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using BRD3 Polyclonal antibody (11859-1-AP)

    WB analysis of COLO 320 using 11859-1-AP (same clone as 11859-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BRD3 Polyclonal antibody (11859-1-AP)

    WB analysis of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using BRD3 Polyclonal antibody (11859-1-AP)

    IP experiment of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    IP result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BRD3 Polyclonal antibody (11859-1-AP)

    IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BRD3 Polyclonal antibody (11859-1-AP)

    IHC staining of human gliomas using 11859-1-AP (same clone as 11859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11859-1-AP (BRD3 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BRD3 Polyclonal antibody (11859-1-AP)

    IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BRD3 Polyclonal antibody (11859-1-AP)

    IF Staining of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11859-1-PBS targets BRD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRD3 fusion protein Ag2433 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 3
Calculated molecular weight 556 aa, 61 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC032124
Gene Symbol BRD3
Gene ID (NCBI) 8019
RRIDAB_2065902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15059
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).