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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRD4 Monoclonal antibody (67374-2-Ig)

    WB analysis using 67374-2-Ig (same clone as 67374-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67374-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using BRD4 Monoclonal antibody (67374-2-Ig)

    WB analysis of NIH/3T3 using 67374-2-Ig (same clone as 67374-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67374-2-Ig (BRD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67374-2-PBS in a different storage buffer formulation.

BRD4 Monoclonal antibody, PBS Only

BRD4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67374-2-PBS
Clone No.2F7E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Synonyms

BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67374-2-PBS targets BRD4 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27545

Product name: Recombinant human BRD4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 311-633 aa of BC035266

Sequence: PEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRV

相同性解析による交差性が予測される生物種
Full Name bromodomain containing 4
Calculated molecular weight 1362 aa, 152 kDa
Observed molecular weight200 kDa
GenBank accession numberBC035266
Gene Symbol BRD4
Gene ID (NCBI) 23476
RRIDAB_2918488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO60885
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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