Featured Product
KD/KO Validated

BRD7 Polyclonal antibody

Name: BRD7 Polyclonal antibody
Brand: Proteintech
SKU: 51009-2-AP

BRD7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 51009-2-AP

Synonyms

75 kDa bromodomain protein, BP75, BRD7, bromodomain containing 7, CELTIX1, NAG4, Protein CELTIX 1

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of HeLa using 51009-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 51009-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 51009-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 51009-2-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 51009-2-AP
L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 51009-2-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 51009-2-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 51009-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 51009-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 51009-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 51009-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens).

-/ -

Cat No. 51009-2-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, mouse cerebellum tissue, mouse lung tissue, L02 cells, rat brain tissue, SH-SY5Y cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, PC-3 cells, SH-5Y5Y cells
Positive IHC detected in	human colon cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 25 publications below
IHC	See 11 publications below
IF	See 6 publications below
IP	See 1 publications below
CoIP	See 2 publications below
ChIP	See 1 publications below

Product Information

51009-2-AP targets BRD7 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BRD7 fusion protein Ag3911 相同性解析による交差性が予測される生物種
Full Name	bromodomain containing 7
Calculated molecular weight	651 aa, 74 kDa
Observed molecular weight	70-74 kDa
GenBank accession number	BC050728
Gene symbol	BRD7
Gene ID (NCBI)	29117
RRID	AB_2259226
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

Protocols

Product Specific Protocols
WB protocol for BRD7 antibody 51009-2-AP	Download protocol
IHC protocol for BRD7 antibody 51009-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Discov A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction. Authors - Jinxiu Li View Article
		Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis Authors - Kaiwen Bao View Article
mouse	IF	Nat Commun SS18 regulates pluripotent-somatic transition through phase separation. Authors - Junqi Kuang View Article
human	WB	Cell Rep FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression Authors - Yanhua Zhang View Article
human	WB,CoIP	Cancer Res BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Authors - Harte Mary T MT KD Validated View Article
mouse	WB	Cell Mol Immunol BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway. Authors - Ran Zhao KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRD7 Polyclonal antibody

BRD7 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 51009-2-AP

WB analysis using 51009-2-AP

WB analysis using 51009-2-AP

WB analysis of rat brain using 51009-2-AP

WB analysis of L02 using 51009-2-AP

WB analysis of mouse cerebellum using 51009-2-AP

WB analysis of mouse lung using 51009-2-AP

WB analysis of SH-SY5Y using 51009-2-AP

IHC staining of human colon cancer using 51009-2-AP

IHC staining of human cervical cancer using 51009-2-AP

IHC staining of human cervical cancer using 51009-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

SS18 regulates pluripotent-somatic transition through phase separation.

FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression

BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription.

BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway.