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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of HeLa using 51009-2-AP (same clone as 51009-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis using 51009-2-AP (same clone as 51009-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis using 51009-2-AP (same clone as 51009-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of SH-SY5Y using 51009-2-AP (same clone as 51009-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of rat brain using 51009-2-AP (same clone as 51009-2-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of L02 using 51009-2-AP (same clone as 51009-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of mouse cerebellum using 51009-2-AP (same clone as 51009-2-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using BRD7 Polyclonal antibody (51009-2-AP)

    WB analysis of mouse lung using 51009-2-AP (same clone as 51009-2-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BRD7 Polyclonal antibody (51009-2-AP)

    IHC staining of human colon cancer using 51009-2-AP (same clone as 51009-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BRD7 Polyclonal antibody (51009-2-AP)

    IHC staining of human cervical cancer using 51009-2-AP (same clone as 51009-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BRD7 Polyclonal antibody (51009-2-AP)

    IHC staining of human cervical cancer using 51009-2-AP (same clone as 51009-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51009-2-PBS targets BRD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRD7 fusion protein Ag3911 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 7
Calculated molecular weight 651 aa, 74 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC050728
Gene Symbol BRD7
Gene ID (NCBI) 29117
RRIDAB_2259226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPI1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.