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  • Featured Product
  • KD/KO Validated

BRD8 Polyclonal antibody

BRD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10476-1-AP

Synonyms

TrCP120, SMAP2, SMAP, p120, Bromodomain-containing protein 8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BRD8 Polyclonal antibody (10476-1-AP)

    WB analysis of HeLa using 10476-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BRD8 Polyclonal antibody (10476-1-AP)

    WB analysis using 10476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BRD8 Polyclonal antibody (10476-1-AP)

    WB analysis of HeLa using 10476-1-AP

    WB result of BRD8 antibody (10476-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRD8 transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using BRD8 Polyclonal antibody (10476-1-AP)

    WB analysis of human brain using 10476-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using BRD8 Polyclonal antibody (10476-1-AP)

    WB analysis of human brain using 10476-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using BRD8 Polyclonal antibody (10476-1-AP)

    IHC staining of human stomach using 10476-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10476-1-AP (BRD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BRD8 Polyclonal antibody (10476-1-AP)

    IF Staining of HeLa using 10476-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRD8 antibody (10476-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, HuH-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10476-1-AP targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRD8 fusion protein Ag0790 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 8
Calculated molecular weight 120 kDa
Observed molecular weight 120-170 kDa
GenBank accession numberBC008039
Gene symbol BRD8
Gene ID (NCBI) 10902
RRIDAB_2066027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)

Protocols

Product Specific Protocols
WB protocol for BRD8 antibody 10476-1-APDownload protocol
IHC protocol for BRD8 antibody 10476-1-APDownload protocol
IF protocol for BRD8 antibody 10476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Authors - Arnaud Obri
View Article
humanWB

Arch Biochem Biophys

BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5.

Authors - Zhaoxiang Yu
View Article