Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, HuH-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
10476-1-AP targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BRD8 fusion protein Ag0790 相同性解析による交差性が予測される生物種 |
| Full Name | bromodomain containing 8 |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 120-170 kDa |
| GenBank accession number | BC008039 |
| Gene Symbol | BRD8 |
| Gene ID (NCBI) | 10902 |
| RRID | AB_2066027 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0E9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine-binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. Interestingly, in the breast cancer cells we detected the 170 kDa endogenous BRD8 protein, which was larger than its calculated 135 kDa molecular weight (MW) from its a.a. residues. The study found that with an anti‐HA tag antibody, we validated the expression of HA‐tagged full‐length BRD8 and four different BRD8 fragments expressed in HEK293T cells. The apparent MWs of these BRD8 full‐length and fragment proteins were also larger than their calculated MWs based on their a.a. residues. (PMID: 37680145)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD8 antibody 10476-1-AP | Download protocol |
| IHC protocol for BRD8 antibody 10476-1-AP | Download protocol |
| IF protocol for BRD8 antibody 10476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arch Biochem Biophys BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5. |