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  • Featured Product
  • KD/KO Validated

BRD8 Monoclonal antibody, PBS Only

BRD8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B6D6

Cat no : 60121-1-PBS

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BRD8 Monoclonal antibody (60121-1-Ig)

    WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

    WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using BRD8 Monoclonal antibody (60121-1-Ig)

    WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BRD8 Monoclonal antibody (60121-1-Ig)

    IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BRD8 Monoclonal antibody (60121-1-Ig)

    IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BRD8 Monoclonal antibody (60121-1-Ig)

    IF Staining of HEK-293 using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HEK-293 cells using 60121-1-Ig(BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BRD8 Monoclonal antibody (60121-1-Ig)

    IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60121-1-PBS targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BRD8 fusion protein Ag0790 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 8
Calculated molecular weight 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC008039
Gene symbol BRD8
Gene ID (NCBI) 10902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].