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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRD9 Monoclonal antibody

BRD9 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C10F2

Cat no : 68922-1-Ig

Synonyms

UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRD9 Monoclonal antibody (68922-1-Ig)

    WB analysis using 68922-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68922-1-Ig (BRD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using BRD9 Monoclonal antibody (68922-1-Ig)

    FC experiment of HeLa using 68922-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 μg BRD9 Monoclonal antibody (68922-1-Ig, Clone:1C10F2, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1), and 0.2 μg Mouse IgG1 isotype Control Antibody (66360-1-Ig, Clone: T1F8D3F10, blue) was stained parallely as control. Cells were fixed with 4% PFA .

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, MDA-MB-231 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68922-1-Ig targets BRD9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BRD9 fusion protein Ag20438 相同性解析による交差性が予測される生物種
Full Name bromodomain containing 9
Calculated molecular weight 597 aa, 67 kDa
Observed molecular weight70 kDa
GenBank accession numberBC041590
Gene symbol BRD9
Gene ID (NCBI) 65980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription.

Protocols

Product Specific Protocols
WB protocol for BRD9 antibody 68922-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols