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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRE Monoclonal antibody, PBS Only

BRE Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5A5

Cat no : 66356-1-PBS

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using BRE Monoclonal antibody (66356-1-Ig)

    WB analysis of fetal human brain using 66356-1-Ig (same clone as 66356-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using BRE Monoclonal antibody (66356-1-Ig)

    WB analysis of rat brain using 66356-1-Ig (same clone as 66356-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BRE Monoclonal antibody (66356-1-Ig)

    IHC staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BRE Monoclonal antibody (66356-1-Ig)

    IHC staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BRE Monoclonal antibody (66356-1-Ig)

    IHC staining of human gliomas using 66356-1-Ig (same clone as 66356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BRE Monoclonal antibody (66356-1-Ig)

    IHC staining of human gliomas using 66356-1-Ig (same clone as 66356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using BRE Monoclonal antibody (66356-1-Ig)

    IF Staining of mouse brain using 66356-1-Ig (same clone as 66356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRE antibody (66356-1-Ig, Clone: 2E5A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66356-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66356-1-PBS targets BRE in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BRE fusion protein Ag2256 相同性解析による交差性が予測される生物種
Full Name brain and reproductive organ-expressed (TNFRSF1A modulator)
Calculated molecular weight 383 aa, 44 kDa
Observed molecular weight 42-47 kDa
GenBank accession numberBC001251
Gene symbol BRE
Gene ID (NCBI) 9577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.