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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of HT-1080 using 12056-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using BRF2 Polyclonal antibody (12056-1-AP)

    WB analysis of DU 145 using 12056-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 12056-1-AP (BRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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BRF2 Polyclonal antibody

BRF2 Polyclonal Antibody for WB, ELISA
Cat No. 12056-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

B related factor 2, BRF 2, BRF2, BRFU, hBRFU, hTFIIIB50, TFIIIB50

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, DU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12056-1-AP targets BRF2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRF2 fusion protein Ag2686 相同性解析による交差性が予測される生物種
Full Name BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC010648
Gene Symbol BRF2
Gene ID (NCBI) 55290
RRIDAB_2218683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human cells contain two types of RNA polymerase III transcription factor(TFIIIB), BRF1 and BRF2. BRF2,also named as BRFU, contains one TFIIB-type zinc finger. It is a general activator of RNA polymerase III transcription. It is a factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.BRF2 is recruited to type 3 promoters such as the human U6 snRNA promoter. It differs from BRF1-TFIIIB in that it contains the TFIIB-related factor BRF2 iinstead of BRF1 and its three components do not form a stable complex. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human BRF2. It recognizes a 50-60kd band in WB analysis.

Protocols

Product Specific Protocols
WB protocol for BRF2 antibody 12056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter

Authors - Jianfeng Sun
View Article