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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of fetal human brain using 60336-1-Ig (same clone as 60336-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of mouse brain using 60336-1-Ig (same clone as 60336-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60336-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of pig brain using 60336-1-Ig (same clone as 60336-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60336-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BRINP1 Monoclonal antibody (60336-1-Ig)

    IF Staining of MCF-7 using 60336-1-Ig (same clone as 60336-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60336-1-Ig(DBC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60336-1-PBS in a different storage buffer formulation.

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BRINP1 Monoclonal antibody, PBS Only

BRINP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60336-1-PBS
Clone No.2A10E3

Host / Isotype

Mouse / IgG2c

Reactivity

human, pig, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60336-1-PBS targets BRINP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen BRINP1 fusion protein Ag6588 相同性解析による交差性が予測される生物種
Full Name deleted in bladder cancer 1
Calculated molecular weight 761 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC021560
Gene Symbol BRINP1
Gene ID (NCBI) 1620
RRIDAB_2881445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60477
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BRINP1, also known as DBC1 and FAM5A, inhibits cell proliferation by negative regulation of the G1/S transition. It mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway.