• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRIP1(C-terminal) Polyclonal antibody

BRIP1(C-terminal) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20983-1-AP

Synonyms

BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using BRIP1(C-terminal) Polyclonal antibody (20983-1-AP)

    WB analysis of HeLa using 20983-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20983-1-AP (BRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20983-1-AP targets BRIP1(C-terminal) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name BRCA1 interacting protein C-terminal helicase 1
Calculated molecular weight 141 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberNM_032043
Gene symbol BACH1/BRIP1
Gene ID (NCBI) 83990
RRIDAB_2878782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRIP1, also named as BACH1 and FANCJ, belongs to the DEAD box helicase family and DEAH subfamily. It is a DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. It is involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Defects in BRIP1 are a cause of susceptibility to breast cancer (BC). Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ). The antibody is specific to BRIP1.

Protocols

Product Specific Protocols
WB protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols