Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 11962-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 11962-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 11962-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11962-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 11962-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 11962-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 11962-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 11962-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11962-1-AP (BRIT1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11962-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 11962-1-AP (BRIT1 Antibody) at dilution of 1:50.

Featured Product
KD/KO Validated

MCPH/BRIT1 Polyclonal antibody

MCPH/BRIT1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11962-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BRIT1, MCPH1, MCT, Microcephalin, microcephalin 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue
Positive IHC detected in	human prostate cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCPH/BRIT1 fusion protein Ag2567 相同性解析による交差性が予測される生物種
Full Name	microcephalin 1
Calculated molecular weight	93 kDa
Observed molecular weight	66 kDa, 100 kDa
GenBank accession number	BC030702
Gene Symbol	MCPH1
Gene ID (NCBI)	79648
RRID	AB_2142989
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NEM0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )

Protocols

Product Specific Protocols
WB protocol for MCPH/BRIT1 antibody 11962-1-AP	Download protocol
IHC protocol for MCPH/BRIT1 antibody 11962-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	FASEB J Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1. Authors - Stephanie K Meyer KD Validated View Article
human	WB, IHC	Stem Cell Res Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology. Authors - Zerui Wang KO Validated View Article
human	WB	FEBS J Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1. Authors - Maria Arroyo KD Validated View Article
human	WB	Genes (Basel) MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II. Authors - María Arroyo KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11962-1-AP

WB analysis of MDA-MB-453s using 11962-1-AP

WB analysis of MDA-MB-453s using 11962-1-AP

WB analysis of L02 using 11962-1-AP

WB analysis of MDA-MB-453s using 11962-1-AP

WB analysis of MDA-MB-453s using 11962-1-AP

WB analysis of mouse ovary using 11962-1-AP

IHC staining of human prostate cancer using 11962-1-AP

IHC staining of human kidney using 11962-1-AP

MCPH/BRIT1 Polyclonal antibody

MCPH/BRIT1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.

Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.

Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.

MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.