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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRP16 Polyclonal antibody

BRP16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20169-1-AP

Synonyms

Brain protein 16, BRP16, C8orf30A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRP16 Polyclonal antibody (20169-1-AP)

    WB analysis using 20169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20169-1-AP (BRP16 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BRP16 Polyclonal antibody (20169-1-AP)

    IF Staining of HepG2 using 20169-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20169-1-AP (BRP16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20169-1-AP targets BRP16 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRP16 fusion protein Ag13998 相同性解析による交差性が予測される生物種
Full Name chromosome 8 open reading frame 30A
Calculated molecular weight 390 aa, 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC003035
Gene symbol BRP16
Gene ID (NCBI) 51236
RRIDAB_2878652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BRP16 antibody 20169-1-APDownload protocol
IF protocol for BRP16 antibody 20169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols