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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BRWD1 Polyclonal antibody

BRWD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29293-1-AP

Synonyms

WDR9, WD repeat-containing protein 9, WD repeat containing protein 9, N143, C21orf107

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29293-1-AP targets BRWD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRWD1 fusion protein Ag30902 相同性解析による交差性が予測される生物種
Full Name bromodomain and WD repeat domain containing 1
Calculated molecular weight 2320 aa, 263 kDa
Observed molecular weight263 kDa
GenBank accession numberBC064602
Gene symbol BRWD1
Gene ID (NCBI) 54014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).

Protocols

Product Specific Protocols
WB protocol for BRWD1 antibody 29293-1-APDownload protocol
IHC protocol for BRWD1 antibody 29293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols