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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BRWD1 Polyclonal antibody (29293-1-AP)

    WB analysis using 29293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29293-1-AP (BRWD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using BRWD1 Polyclonal antibody (29293-1-AP)

    IHC staining of mouse cerebellum using 29293-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29293-1-AP (BRWD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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BRWD1 Polyclonal antibody

BRWD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29293-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

WDR9, WD repeat-containing protein 9, WD repeat containing protein 9, N143, C21orf107

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29293-1-AP targets BRWD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRWD1 fusion protein Ag30902 相同性解析による交差性が予測される生物種
Full Name bromodomain and WD repeat domain containing 1
Calculated molecular weight 2320 aa, 263 kDa
Observed molecular weight263 kDa
GenBank accession numberBC064602
Gene Symbol BRWD1
Gene ID (NCBI) 54014
RRIDAB_3086110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRWD1 (Bromodomain and WD repeat domain containing 1, also known as WDR9) has a predicted molecular weight of 263 kDa and contains tandem BROMO domains and WD40 repeats (PMID:12889071). One of the remarkable functions of BRWD1 was the coordinated repression of MYC and MYC target genes. At the Myc/MYC locus, in both mice and humans, BRWD1 specififically repressed distal enhancers, which are associated with lineage specifific expression (PMID:30250168). In addition,BRWD1 as a key epigenomic mediator of normal neurodevelopment and an important contributor to DS (Down syndrome)-related phenotypes (PMID:36289231).

Protocols

Product Specific Protocols
WB protocol for BRWD1 antibody 29293-1-APDownload protocol
IHC protocol for BRWD1 antibody 29293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols