Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IP detected in | Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IP | See 1 publications below |
Product Information
26709-1-AP targets BSAP,PAX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BSAP,PAX5 fusion protein Ag25147 相同性解析による交差性が予測される生物種 |
| Full Name | paired box 5 |
| Calculated molecular weight | 391 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC156927 |
| Gene Symbol | PAX5 |
| Gene ID (NCBI) | 5079 |
| RRID | AB_2880608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02548 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BSAP,PAX5 antibody 26709-1-AP | Download protocol |
| IHC protocol for BSAP,PAX5 antibody 26709-1-AP | Download protocol |
| IP protocol for BSAP,PAX5 antibody 26709-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis. | ||
J Cutan Pathol Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases. | ||
Mol Oncol miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer | ||
Free Radic Biol Med Prohibitin 2 confers NADPH oxidase 1 -mediated cytosolic oxidative signaling to promote gastric cancer progression by ERK activation | ||
Cell Mol Immunol Rack1 regulates B-cell development and function by binding to and stabilizing the transcription factor Pax5 |