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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BSEP Monoclonal antibody, PBS Only

BSEP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3C11D5

Cat no : 67512-1-PBS

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BSEP Monoclonal antibody (67512-1-Ig)

    WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    WB analysis of rat liver using 67512-1-Ig (same clone as 67512-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig lung tissue using BSEP Monoclonal antibody (67512-1-Ig)

    WB analysis of pig lung using 67512-1-Ig (same clone as 67512-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BSEP Monoclonal antibody (67512-1-Ig)

    WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    Unboiled HepG2 cell lysates, unboiled A549 cell lysates, unboilled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IHC staining of human cervical cancer using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IHC staining of human cervical cancer using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:10000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using BSEP Monoclonal antibody (67512-1-Ig)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67512-1-PBS targets BSEP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BSEP fusion protein Ag29135 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Calculated molecular weight 146 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberNM_003742
Gene symbol BSEP
Gene ID (NCBI) 8647
RRIDAB_2882733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)