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  • KD/KO Validated

BST2 Polyclonal antibody

BST2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13560-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

CD317, Tetherin, CD 317, BST-2, BST 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13560-1-AP targets BST2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 30-36 kDa, 50-70 kDa
GenBank accession numberBC033873
Gene Symbol BST2
Gene ID (NCBI) 684
RRIDAB_2067220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks retrovirus release from the cell surface. Depleted upon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane protein, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 are 30-36 kDa (monomer form) and 50-70 kDa (dimer form) (PMID: 19196977; 21237475; 19737401; 37922253).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 13560-1-APDownload protocol
IHC protocol for BST2 antibody 13560-1-APDownload protocol
IF protocol for BST2 antibody 13560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

PMID: 28965816

Authors - Shouheng Jin
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

PMID: 24753610

Authors - Xuesen Zhao
humanWB, IF

Curr Biol

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.

Authors - Adrien Presle
  • KO Validated
humanWB

PLoS Pathog

PMID: 28910400

Authors - Yuxuan Fu
  • KO Validated
humanWB, IF

J Virol

Interferon-inducible Protein Tetherin Inhibits Hepatitis B Virus Virion Secretion.

Authors - Ran Yan
  • KD Validated
humanWB

J Virol

Interferon-induced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms.

Authors - Weidner Jessica M JM
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