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  • Featured Product
  • KD/KO Validated

BST2 Polyclonal antibody, PBS Only

BST2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13560-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BST2 Polyclonal antibody (13560-1-AP)

    WB analysis using 13560-1-AP (same clone as 13560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13560-1-AP (BST2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BST2 Polyclonal antibody (13560-1-AP)

    WB analysis of HeLa using 13560-1-AP (same clone as 13560-1-PBS)

    WB result of BST2 antibody (13560-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells. This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using BST2 Polyclonal antibody (13560-1-AP)

    IHC staining of human liver using 13560-1-AP (same clone as 13560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13560-1-AP (BST2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using BST2 Polyclonal antibody (13560-1-AP)

    IF Staining of mouse eye using 13560-1-AP (same clone as 13560-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (13560-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using BST2 Polyclonal antibody (13560-1-AP)

    IF Staining of mouse eye using 13560-1-AP (same clone as 13560-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (13560-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using BST2 Polyclonal antibody (13560-1-AP)

    IF Staining of NCCIT using 13560-1-AP (same clone as 13560-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using BST2 antibody (13560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13560-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13560-1-PBS targets BST2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 30-36 kDa, 50-70 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks retrovirus release from the cell surface. Depleted upon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane protein, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 are 30-36 kDa (monomer form) and 50-70 kDa (dimer form) (PMID: 19196977; 21237475; 19737401; 37922253).