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BST2 Polyclonal antibody

BST2 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30118-1-AP

Synonyms

Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BST2 Polyclonal antibody (30118-1-AP)

    WB analysis of HeLa using 30118-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30118-1-AP (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BST2 Polyclonal antibody (30118-1-AP)

    WB analysis of HeLa using 30118-1-AP

    WB result of BST2 antibody (30118-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using BST2 Polyclonal antibody (30118-1-AP)

    IF Staining of mouse eye using 30118-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (30118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using BST2 Polyclonal antibody (30118-1-AP)

    IF Staining of mouse eye using 30118-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using BST2 antibody (30118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30118-1-AP targets BST2 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BST2 fusion protein Ag32333 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 30-36 kDa, 50-70 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 30118-1-APDownload protocol
IF protocol for BST2 antibody 30118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols