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BST2 Monoclonal antibody

BST2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C4B5

Cat no : 66919-1-Ig

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis of HeLa using 66919-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis using 66919-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis of HeLa using 66919-1-Ig

    WB result of BST2 antibody (66919-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BST2 transfected HeLa cells.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66919-1-Ig

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis of HepG2 using 66919-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using BST2 Monoclonal antibody (66919-1-Ig)

    WB analysis of L02 using 66919-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66919-1-Ig (BST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using BST2 Monoclonal antibody (66919-1-Ig)

    IHC staining of human liver using 66919-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using BST2 Monoclonal antibody (66919-1-Ig)

    IHC staining of human liver using 66919-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66919-1-Ig (BST2 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BST2 Monoclonal antibody (66919-1-Ig)

    IF Staining of HeLa using 66919-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BST2 antibody (66919-1-Ig, Clone: 3C4B5 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human peripheral blood leukocyte, L02 cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:1250-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66919-1-Ig targets BST2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BST2 fusion protein Ag4430 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 18 kDa, 30-36 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
RRIDAB_2882246
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 66919-1-IgDownload protocol
IHC protocol for BST2 antibody 66919-1-IgDownload protocol
IF protocol for BST2 antibody 66919-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Mol Biol Cell

ER-to-Golgi trafficking of procollagen III via conventional vesicular and tubular carriers.

Authors - Yukihiro Hirata
View Article