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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BST2 Recombinant antibody

BST2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241513A1

Cat no : 84190-7-RR

Synonyms

CD317, CD 317, BST-2, BST 2, Bone marrow stromal antigen 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BST2 Recombinant antibody (84190-7-RR)

    WB analysis using 84190-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84190-7-RR (BST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BST2 Recombinant antibody (84190-7-RR)

    IF Staining of HeLa using 84190-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BST2 antibody (84190-7-RR, Clone: 241513A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84190-7-RR

    Affinity and Kinetic Characterization of 84190-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84190-7-RR against Human BST2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, THP-1 cells, human heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84190-7-RR targets BST2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 2
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight 30-36 kDa, 50-70 kDa
GenBank accession numberBC033873
Gene symbol BST2
Gene ID (NCBI) 684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).

Protocols

Product Specific Protocols
WB protocol for BST2 antibody 84190-7-RRDownload protocol
IF protocol for BST2 antibody 84190-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols