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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis using 66683-1-Ig (same clone as 66683-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of NCCIT using 66683-1-Ig (same clone as 66683-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of A549 using 66683-1-Ig (same clone as 66683-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of MDA-MB-231 using 66683-1-Ig (same clone as 66683-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of T-47D using 66683-1-Ig (same clone as 66683-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using Betacellulin Monoclonal antibody (66683-1-Ig)

    WB analysis of PC-3 using 66683-1-Ig (same clone as 66683-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66683-1-Ig (BTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66683-1-PBS in a different storage buffer formulation.

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Betacellulin Monoclonal antibody, PBS Only

Betacellulin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66683-1-PBS
Clone No.2H5E8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BTC, Probetacellulin, 2H5E8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66683-1-PBS targets Betacellulin in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Betacellulin fusion protein Ag26724 相同性解析による交差性が予測される生物種
Full Name betacellulin
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011618
Gene Symbol BTC
Gene ID (NCBI) 685
RRIDAB_2882037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35070
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.