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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTD Polyclonal antibody

BTD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16330-1-AP

Synonyms

EC:3.5.1.12, biotinidase, Biotinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BTD Polyclonal antibody (16330-1-AP)

    WB analysis using 16330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16330-1-AP (BTD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using BTD Polyclonal antibody (16330-1-AP)

    IHC staining of mouse kidney using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using BTD Polyclonal antibody (16330-1-AP)

    IHC staining of human liver using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using BTD Polyclonal antibody (16330-1-AP)

    IHC staining of human liver using 16330-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BTD Polyclonal antibody (16330-1-AP)

    IF Staining of HeLa using 16330-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTD antibody (16330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16330-1-AP targets BTD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTD fusion protein Ag9449 相同性解析による交差性が予測される生物種
Full Name biotinidase
Calculated molecular weight 543 aa, 61 kDa
Observed molecular weight61 kDa
GenBank accession numberBC012099
Gene symbol BTD
Gene ID (NCBI) 686
RRIDAB_2067512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

Protocols

Product Specific Protocols
WB protocol for BTD antibody 16330-1-APDownload protocol
IHC protocol for BTD antibody 16330-1-APDownload protocol
IF protocol for BTD antibody 16330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols