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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTG4 Polyclonal antibody

BTG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20514-1-AP

Synonyms

B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BTG4 Polyclonal antibody (20514-1-AP)

    WB analysis using 20514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20514-1-AP (BTG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BTG4 Polyclonal antibody (20514-1-AP)

    WB analysis of HeLa using 20514-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20514-1-AP (BTG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using BTG4 Polyclonal antibody (20514-1-AP)

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BTG4 Polyclonal antibody (20514-1-AP)

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BTG4 Polyclonal antibody (20514-1-AP)

    IHC staining of human testis using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BTG4 Polyclonal antibody (20514-1-AP)

    IHC staining of human testis using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20514-1-AP is BTG4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTG4 fusion protein Ag14339 相同性解析による交差性が予測される生物種
Full Name B-cell translocation gene 4
Calculated molecular weight 223 aa, 26 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC031045
Gene symbol BTG4
Gene ID (NCBI) 54766
RRIDAB_10694822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BTG4 antibody 20514-1-APDownload protocol
IHC protocol for BTG4 antibody 20514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols