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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTLA Polyclonal antibody

BTLA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21300-1-AP

Synonyms

B and T lymphocyte attenuator, B and T lymphocyte associated, BTLA, BTLA1, CD272

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using BTLA Polyclonal antibody (21300-1-AP)

    WB analysis of Raji using 21300-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21300-1-AP (BTLA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BTLA Polyclonal antibody (21300-1-AP)

    WB analysis of Jurkat using 21300-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21300-1-AP (BTLA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BTLA Polyclonal antibody (21300-1-AP)

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BTLA Polyclonal antibody (21300-1-AP)

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 21300-1-AP is BTLA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTLA fusion protein Ag15884 相同性解析による交差性が予測される生物種
Full Name B and T lymphocyte associated
Calculated molecular weight 289 aa, 33 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC107091
Gene symbol BTLA
Gene ID (NCBI) 151888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanIHC

Onco Targets Ther

BTLA Expression in Stage I-III Non-Small-Cell Lung Cancer and Its Correlation with PD-1/PD-L1 and Clinical Outcomes.

Authors - Xiangmin Li
View Article