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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using BTLA Polyclonal antibody (21300-1-AP)

    WB analysis of Raji using 21300-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21300-1-AP (BTLA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BTLA Polyclonal antibody (21300-1-AP)

    WB analysis of Jurkat using 21300-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21300-1-AP (BTLA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BTLA Polyclonal antibody (21300-1-AP)

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BTLA Polyclonal antibody (21300-1-AP)

    IHC staining of human tonsillitis using 21300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21300-1-AP (BTLA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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BTLA Polyclonal antibody

BTLA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21300-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

B and T lymphocyte attenuator, B and T lymphocyte associated, BTLA, BTLA1, CD272

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

21300-1-AP targets BTLA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTLA fusion protein Ag15884 相同性解析による交差性が予測される生物種
Full Name B and T lymphocyte associated
Calculated molecular weight 289 aa, 33 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC107091
Gene Symbol BTLA
Gene ID (NCBI) 151888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6A9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanIHC

Onco Targets Ther

BTLA Expression in Stage I-III Non-Small-Cell Lung Cancer and Its Correlation with PD-1/PD-L1 and Clinical Outcomes.

Authors - Xiangmin Li
View Article