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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BTLA Recombinant antibody (84754-5-RR)

    WB analysis using 84754-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84754-5-RR (BTLA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A20 cells using BTLA Recombinant antibody (84754-5-RR)

    IF Staining of A20 using 84754-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A20 cells using BTLA antibody (84754-5-RR, Clone: 242133E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84754-5-RR

    Affinity and Kinetic Characterization of 84754-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84754-5-RR against Mouse BTLA were performed. The affinity constant is 1.12 nM.

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BTLA Recombinant antibody

BTLA Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84754-5-RR
Clone No.242133E5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CD272, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133E5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IF/ICC detected inA20 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84754-5-RR targets BTLA in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name B and T lymphocyte associated
Calculated molecular weight34kDa
Observed molecular weight50 kDa
GenBank accession numberAAI08964.1
Gene Symbol Btla
Gene ID (NCBI) 208154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ32MV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTLA, or B and T lymphocyte attenuator, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

Protocols

Product Specific Protocols
WB protocol for BTLA antibody 84754-5-RRDownload protocol
IF protocol for BTLA antibody 84754-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols