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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human BTLA/CD272 Rabbit Recombinant Antibody, PBS Only

BTLA/CD272 Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241318H5

Cat no : 98096-1-PBS

Synonyms

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01168-2

    Cytometric bead array standard curve of MP01168-2

    Cytometric bead array standard curve of MP01168-2, BTLA/CD272 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98096-1-PBS. Detection antibody: 84262-2-PBS. Standard: RP02326. Range: 0.078-10 ng/mL.

  • Affinity and Kinetic Characterization of 98096-1-RR

    Affinity and Kinetic Characterization of 98096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98096-1-PBS targets BTLA/CD272 as part of a matched antibody pair:

MP01168-2: 98096-1-PBS capture and 84262-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name B and T lymphocyte associated
Calculated molecular weight 289 aa, 33 kDa
GenBank accession numberBC107091
Gene symbol BTLA
Gene ID (NCBI) 151888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.