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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTN2A1 Polyclonal antibody

BTN2A1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20596-1-AP

Synonyms

BK14H9.1, BT2.1, BTF1, BTN2A1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using BTN2A1 Polyclonal antibody (20596-1-AP)

    WB analysis of Jurkat using 20596-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using BTN2A1 Polyclonal antibody (20596-1-AP)

    WB analysis of mouse spleen using 20596-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using BTN2A1 Polyclonal antibody (20596-1-AP)

    WB analysis of rat spleen using 20596-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BTN2A1 Polyclonal antibody (20596-1-AP)

    WB analysis of HeLa using 20596-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BTN2A1 Polyclonal antibody (20596-1-AP)

    WB analysis of MCF-7 using 20596-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20596-1-AP (BTN2A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, mouse spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20596-1-AP is BTN2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTN2A1 fusion protein Ag14545 相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A1
Calculated molecular weight 60 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC016661
Gene symbol BTN2A1
Gene ID (NCBI) 11120
RRIDAB_10693536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)

Protocols

Product Specific Protocols
WB protocol for BTN2A1 antibody 20596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols