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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BTN2A1 Monoclonal antibody (68265-1-Ig)

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using BTN2A1 Monoclonal antibody (68265-1-Ig)

    WB analysis using 68265-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68265-1-Ig (BTN2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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BTN2A1 Monoclonal antibody

BTN2A1 Monoclonal Antibody for WB, ELISA
Cat No. 68265-1-Ig
Clone No.3A6G2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

BK14H9.1, BT2.1, BTF1, BTN2A1, DJ3E1.1, FLJ36567

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68265-1-Ig targets BTN2A1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BTN2A1 fusion protein Ag14533 相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A1
Calculated molecular weight 60 kDa
Observed molecular weight69 kDa
GenBank accession numberBC016661
Gene Symbol BTN2A1
Gene ID (NCBI) 11120
RRIDAB_2935350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7KYR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 69 kDa. (PMID: 17785817)

Protocols

Product Specific Protocols
WB protocol for BTN2A1 antibody 68265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols