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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTN2A1 Recombinant antibody

BTN2A1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241540F10

Cat no : 84550-1-RR

Synonyms

BTF1, BT2.1, 241540F10

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BTN2A1 Recombinant antibody (84550-1-RR)

    WB analysis of HeLa using 84550-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BTN2A1 Recombinant antibody (84550-1-RR)

    WB analysis using 84550-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84550-1-RR (BTN2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84550-1-RR

    Affinity and Kinetic Characterization of 84550-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84550-1-RR against Human BTN2A1 were performed. The affinity constant is 35.9 nM.

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Tested Applications

Positive WB detected inHeLa cells, THP-1 cells, MCF-7 cells, NCCIT cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84550-1-RR is BTN2A1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A1
Calculated molecular weight 60 kDa
Observed molecular weight65 kDa
GenBank accession numberBC016661
Gene symbol BTN2A1
Gene ID (NCBI) 11120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTN2A1, also named as BT2.1, BTF1 is a 527 amino acid protein, which belongs to the immunoglobulin superfamily. BTN2A1 is highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas and moderate expression was seen in lung, liver and kidney. The calcualted molecular weight of BTN2A1 is 59 kDa, but glycosylation of BTN2A1 is about 65-69 kDa. (PMID: 17785817)

Protocols

Product Specific Protocols
WB protocol for BTN2A1 antibody 84550-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols