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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BTN2A2 Polyclonal antibody

BTN2A2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16482-1-AP

Synonyms

BTF2, BT2.2

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of MCF-7 using 16482-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of human testis using 16482-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    WB analysis of NIH/3T3 using 16482-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16482-1-AP (BTN2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BTN2A2 Polyclonal antibody (16482-1-AP)

    IF Staining of HepG2 using 16482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BTN2A2 antibody (16482-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, NIH/3T3 cells, MCF-7 cells, human testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16482-1-AP targets BTN2A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTN2A2 fusion protein Ag9603 相同性解析による交差性が予測される生物種
Full Name butyrophilin, subfamily 2, member A2
Calculated molecular weight 523 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC014021
Gene symbol BTN2A2
Gene ID (NCBI) 10385
RRIDAB_2228110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Butyrophilins constitute a family of transmembrane proteins consisting of butyrophilin (BTN), BTN-like (BTNL), and selection and upkeep of intraepithelial T cell (SKI NT) proteins. It has been reported that BTN2A2 is expressed on B cells, DCs, and macrophages and the expression levels are upregulated upon activation. BTN2A2 is also expressed on thymic epithelial cells (TECs). The BTN2A2 putative receptor is expressed on activated T cells. BTN2A2 protein inhibits T cell proliferation, metabolism and Th1 cytokine production(PMID: 34588505). The calculated MW of BTN2A2 is 59kDa, and the glycosylated form is around 65kDa (PMID:20208008).

Protocols

Product Specific Protocols
WB protocol for BTN2A2 antibody 16482-1-APDownload protocol
IF protocol for BTN2A2 antibody 16482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols